Characteristics of the processes by which steroid and thyroid hormones enter tissues, cells and membrane vesicles are reviewed. Several authors suggest that entry is by passive diffusion: the accumulation within cells is attributed to cytoplasmic binding proteins. Other authors, however, propose a membrane-mediated process of entry. The involvement of saturability, high specificity, sensitivity to temperature, sulfhydryl and cell-surface-perturbing reagents and hydrolytic enzymes support the latter view. Purified plasma-membrane vesicle preparations retain several characteristics of entry shown by intact cells. Intracellular hormone-binding proteins would not contribute to processes observed with these preparations.
Products with Single-Use Tubes and Applicators: Tear open one cleansing wipe packet (if the product kit contains such item), gently clean the affected area and discard the used cleansing wipe. Remove the child-resistant cap and foil seal from one tube and firmly screw one applicator onto the tube. Do not over tighten. Squeeze the tube to fill the applicator until a small amount of cream/gel comes out of and lubricates the applicator openings. Gently insert the applicator tip with attached tube into anal area. Continue squeezing the body of the tube as it is moved around the areas of discomfort, and lastly, around and in the anal opening (if directed by physician).