Thin-layer chromatographic analysis of steroids a review

Different molecules are carried up the plate to different distances due to variable interactions with the adsorbent material. For example, when silica is used, polar molecules with groups such as hydroxy (OH) or amine (NH 2 ) will tend to form hydrogen bonds with the silica matrix (Si-OH) groups and will therefore not move as fast up the plate. While relatively non-polar molecules will have fewer interactions with the matrix and will tend to be more soluble in the solvent phase and therefore rise faster up with the solvent front ( see animation below )

At the Earth's surface, ozone comes into direct contact with life-forms and displays its destructive side (hence, it is often called "bad ozone"). Because ozone reacts strongly with other molecules, high levels of ozone are toxic to living systems. Several studies have documented the harmful effects of ozone on crop production, forest growth, and human health. The substantial negative effects of surface-level tropospheric ozone from this direct toxicity contrast with the benefits of the additional filtering of UV-B radiation that it provides.

Thin-layer chromatographic analysis of steroids a review

thin-layer chromatographic analysis of steroids a review

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